An Enzymatic Method for Glucose Quantitation in Normal Urine.
نویسنده
چکیده
1 (JRING INVESTIGATIONS of the renal reabsorptive mechanism for glucose, a method to quantitate adequately the glucose of normal urine (1-3) was required. The nonspecificity of classic methods for reducing sugars makes them inadequate for application to normal urine. Specific glucose oxidase methods have not yielded adequate recoveries when applied to normal, dilute urine. I have adopted the enzymatic method for glucose mentioned by Slein et at. (4) and by Con and Lamer (5), which uses hexokinase and glucose-6-phosphate dehydrogenase. The method is here described in detail as used in this laboratory, and its application to dog and human urine is evaluated. The method involves the following 2 reactions:
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عنوان ژورنال:
- Clinical chemistry
دوره 11 شماره
صفحات -
تاریخ انتشار 1965